Porphyromonas gingivalis가 일부 구강미생물의 형광 발현에 미치는 영향
Red fluorescence of oral bacteria interacting with Porphyromonas gingivalis
김세연, 우동협, 이민아, 김지수, 이정하, 정승화,
소속 상세정보
김세연 ( Kim Se-Yeon ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
우동협 ( Woo Dong-Hyeob ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
이민아 ( Lee Min-Ah ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
김지수 ( Kim Ji-Soo ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
이정하 ( Lee Jung-Ha ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
정승화 ( Jeong Seung-Hwa ) - 부산대학교 치의학전문대학원 예방과사회치의학교실
KMID : 0355420170410010022
Abstract
Objectives: Dental plaque is composed of 700 bacterial species. It is known that some oral microorganisms produce porphyrin, and thus, they emit red fluorescence when illuminated with blue light at a specific wavelength of <410 nm. Porphyromonas gingivalis belongs to the genus Porphyromonas, which is characterized by the production of porphyrin. The aim of this study was to evaluate red fluorescence emission of some oral microorganisms interacting with P. gingivalis.
Methods: Five bacterial strains (P. gingivalis, Streptococcus mutans, Lactobacillus casei, Actinomyces naeslundii, and Fusobacterium nucleatum) were used for this study. Tryptic soy agar medium supplemented with hemin, vitamin K3, and sheep blood was used as a growth medium. The fluorescence emission of bacterial colonies was evaluated under 405 nm-wavelength blue light using a Quantitative Light-induced Fluorescence Digital (QLF-D) camera system. Each bacterium was cultured alone and co-cultured in close proximity with P. gingivalis. The red/green (R/G) ratio of fluorescence image was cal-culated and the differences of R/G ratio according to each growth condition were compared using the Mann-Whitney test (P<0.05).
Results: Single cultured S. mutans, L. casei and A. naeslundii colonies emitted red fluorescence (R/G ratio=2.15±0.06, 4.31±0.17, 5.52±1.29, respectively). Fusobacterium nucleatum colonies emitted green fluorescence (R/G ratio=1.36±0.06). The R/G ratios of A. naeslundii and F. nucleatum were increased when P. gingivalis was co-cultured with each bacterium (P<0.05). In contrast, the R/G ratios of S. mutans and L. casei were decreased when P. gingivalis was co-cultured with each bacterium (P=0.002, 0.003).
Conclusions: This study confirmed that P. gingivalis could affect the red fluorescence of other oral bacteria under 405 nm-wavelength blue light. Our findings concluded that P. gingivalis has an important role for red fluorescence emission of dental biofilm.
키워드
Actinomyces naeslundii; Fluorescence; Fusobacterium nucleatum; Lactobacillus casei; Porphyromonas gingivalis; Red fluorescence; Streptococcus mutans
원문 및 링크아웃 정보
등재저널 정보